Fig. 5: Investigation of senescent induction in hAMSC after 24 h of exposure to increasing BPA concentrations. | Cell Death Discovery

Fig. 5: Investigation of senescent induction in hAMSC after 24 h of exposure to increasing BPA concentrations.

From: Bisphenol-A disrupts mitochondrial functionality leading to senescence and apoptosis in human amniotic mesenchymal stromal cells

Fig. 5

β-galactosidase (β-gal) activity in hAMSC was evaluated by immunofluorescence analysis 24 h after exposure to increasing concentrations of BPA (0.1, 0.2, 0.3, and 0.4 μM) (A). β-gal-positive cells were identified by a green signal. Pictures were acquired at ×20 magnification (scale bar corresponds to 100 μM in A). The percentage of senescent cells ((β-gal) positive) detected by immunofluorescence analysis was represented. Expression of senescent-associated secretory phenotype (SASP) molecules (IL-6, IL-1α and CCL20) were analysed by RT-PCR 24 h after BPA exposure and presented as fold-change relative to the control (MetOH) (B). Results are represented as histograms showing mean values ± SD from n = 3 independent experiments. Statistical analysis was performed versus the control condition: p < 0.01(**), p < 0.001(***), p < 0.0001(****).

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