Fig. 6: Investigation of apoptosis induction in hAMSC after 3, 24 and 48 h of exposure to increasing BPA concentrations. | Cell Death Discovery

Fig. 6: Investigation of apoptosis induction in hAMSC after 3, 24 and 48 h of exposure to increasing BPA concentrations.

From: Bisphenol-A disrupts mitochondrial functionality leading to senescence and apoptosis in human amniotic mesenchymal stromal cells

Fig. 6

Percentage of live, early and late apoptotic cells was measured by Annexin-PI fluorogenic dyes 3, 24 and 48 h after exposure to increasing concentrations of BPA (0.05, 0.1, 0.2, 0.3, 0.35, and 0.4 μM) (A). Expression of pro-apoptotic (Bax) and anti-apoptotic (Bcl-2) genes was analysed by RT-PCR 24 h after BPA exposure and presented as fold-change relative to the control condition (MetOH) (B). Results are represented as histograms showing mean values ± SD from n = 3 independent experiments. Statistical analysis was performed versus the control condition: p < 0.01(**), p < 0.001(***), p < 0.0001(****).

Back to article page