Fig. 4: Immunofluorescence for detection and quantification of cytochrome C and calnexin. | Cell Death Discovery

Fig. 4: Immunofluorescence for detection and quantification of cytochrome C and calnexin.

From: CDK inhibitors promote neuroblastoma cell differentiation and increase sensitivity to retinoic acid—a promising combination strategy for therapeutic intervention

Fig. 4

A, B NB cells (LAN-1, CHLA-90) were stained with respective antibodies to determine the subcellular localization of cytochrome C (green), and calnexin (red). The cytoskeleton was visualized using phalloidin green, nuclei were stained with DAPI (blue). Images were taken on a Zeiss AxiovertA.1 Microscope. A, B Representative single channel and merged images. C Quantification of cytochrome C and calnexin intensity. Mean + SD; n = 3 biological replicates. One-way ANOVA (Tukey’s multiple comparisons test). **p < 0.01 vs. ctrl; ##p < 0.01 vs. monotherapy. (B, C) Doses: RA (all cell lines): 1.5 µM; LAN-1 abemaciclib: 0.2 µM; fadraciclib: 0.4 µM; CHLA-90: abemaciclib: 1.0 µM: fadraciclib: 1 µM.

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