Fig. 5: Detection of cell cycle arrest by p27 immunofluorescence and cell death by flow cytometry. | Cell Death Discovery

Fig. 5: Detection of cell cycle arrest by p27 immunofluorescence and cell death by flow cytometry.

From: CDK inhibitors promote neuroblastoma cell differentiation and increase sensitivity to retinoic acid—a promising combination strategy for therapeutic intervention

Fig. 5: Detection of cell cycle arrest by p27 immunofluorescence and cell death by flow cytometry.The alternative text for this image may have been generated using AI.

A, B p27 was detected upon staining with an Alexa Fluor® 488 anti-p27/Kip1 Antibody. A Representative microscopic images of NB cells (LAN-1, CHLA-90), taken on a Zeiss AxiovertA.1 Microscope. B p27 quantification. Mean + SD; n = 3 biological replicates. One-way ANOVA (Tukey’s multiple comparisons test). *p < 0.05. C, D Cell cycle analysis of lentivirally-transduced LAN-1 cells. C Visualization of cell cycle phases by fluorescence microscopy. D Quantification of cell cycle phases. n = 3 biological replicates. Two-way ANOVA (Tukey’s multiple comparisons test). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. ctrl. A–D Doses: RA (both cell lines): 1.5 µM; LAN-1 abemaciclib: 0.2 µM; fadraciclib: 0.4 µM; CHLA-90: abemaciclib: 1.0 µM: fadraciclib: 1 µM.

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