Fig. 8: ADA in combination with PAN elicits significant synthetic lethality in NSCLC cells.

A H23 cells were treated with ADA (0.3125 μM) alone or in combination with BRU (10 nM), PAN (20 nM) and VOR (0.5 μM) for 24 h, after which cell viability was assessed. B–E H23 and H1299 cells were treated with PAN alone or in combination with various concentrations of ADA (20, 40, 80, 160, 320 nM) for 24 h, after which cell viability and related CI values were assessed. F–H H23 and H1299 cells were treated with ADA or PAN alone, or in combination for 3 h, the associated proteins were detected by Western blot. I H1299 cells were treated with ADA or PAN alone, or in combination for 9 h, the associated proteins were detected by Western blot. J, K H23 and H1299 cells were transfected with lentivirus, images were captured using a fluorescence microscope after treated with ADA or PAN alone, or in combination for 9 h. Scale bar = 25 μm. **p < 0.01, as determined by Two-way ANOVA.