Fig. 3: Transcriptome sequencing analyzed the effect of DCTPP1-KD in HTR8/SVneo cells.
From: DCTPP1 regulates oxidative stress homeostasis via AUF1 in human villous trophoblasts
A Cluster analysis plot of differentially expressed genes (DEGs) after DCTPP1 knockdown. C1, C2, C3 and T1, T2, T3 indicate independent data replicates for each group. B Volcanic map analysis of DEGs in DCTPP1-KD and control groups. Differential genes are color coded. C KEGG enrichment analysis enriched DEGs factors after DCTPP1 knockdown. D The GO analysis showing the function linked to the DEGs. E Validation of RNA-seq data related to cell cycle between DCTPP1-KD and control groups using qRT-PCR. F Validation of RNA-seq data related to ROS in DCTPP1-KD and control groups using qRT-PCR. G Validation of RNA-seq data related to apoptosis in DCTPP1-KD and control groups using qRT-PCR. H Representative western blot images showing TP53I3 expression in DCTPP1-KD cells when compared to controls, with GAPDH serving as the loading control. I Quantitative analysis of TP53I3 protein expression. *P < 0.05, **P < 0.01, and ***P < 0.001.
