Fig. 6: Transcriptome sequencing analyzed the effect of AUF1-KD in HTR8/SVneo cells.
From: DCTPP1 regulates oxidative stress homeostasis via AUF1 in human villous trophoblasts
A Cluster analysis plot of DEGs after AUF1 knockdown. C1, C2, C3 and T1, T2, T3 indicate independent data replicates for each group. B Volcanic map analysis of DEGs in AUF1-KD and control groups. Differential genes are color coded. C KEGG analysis enriched DEGs after AUF1 knockdown. D The GO analysis showing the function linked to the DEGs. E Validation of RNA-seq data related to the cell cycle between the AUF1-KD and control groups using qRT-PCR. F Validation of RNA-seq data related to ROS between AUF1-KD and control groups using qRT-PCR. G Validation of RNA-seq data related to apoptosis in AUF1-KD and control groups using qRT-PCR. H The western blot images of TP53I3 and GAPDH in AUF1-KD and control cells. I Bar graph showing relative TP53I3 protein expression (normalized to GAPDH). *P < 0.05, **P < 0.01, and ***P < 0.001.
