Fig. 2: MUC1-C activates type I IFN signaling that induces A3A expression. | Cell Death Discovery

Fig. 2: MUC1-C activates type I IFN signaling that induces A3A expression.

From: Activation of APOBEC3 cytidine deaminases and endogenous retroviruses is integrated by MUC1-C in NSCLC cells

Fig. 2: MUC1-C activates type I IFN signaling that induces A3A expression.The alternative text for this image may have been generated using AI.

H1975 (A) and PC9 (B) cells treated with 1 μM OSI for 3 days were analyzed for STAT1, STAT2 and IRF9 mRNA levels by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for control cells (assigned a value of 1). C Chromatin from H1975 cells treated with 1 μM OSI for 3 days was immunoblotted with antibodies against the indicated proteins. D Soluble chromatin from H1975 cells treated with 1 μM OSI for 3 days was precipitated with antibodies against MUC1-C, STAT1, STAT2 and IRF9. The DNA samples were amplified by qPCR with primers for the MUC1 dELS region. The results (mean ± SD of 4 determinations) are expressed as % input. E, F H1975/tet-MUC1shRNA cells treated with 1 μM OSI for 3 days and vehicle or DOX for 7 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of four determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1) (E). Lysates were immunoblotted with antibodies against the indicated proteins (F). G, H PC9/tet-MUC1shRNA cells treated with 1 μM OSI for 3 days and vehicle or DOX for 7 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of four determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1) (G). Lysates were immunoblotted with antibodies against the indicated proteins (H). I Schema of A3A with highlighting of the pELS region containing a U-ISGF3 binding motif. Soluble chromatin from H1975/tet-MUC1shRNA cells treated with 1 μM OSI for 3 days and vehicle or DOX was precipitated with antibodies against MUC1-C, STAT1, STAT2 and IRF9. The DNA samples were amplified by qPCR with primers for the A3A pELS region. The results (mean ± SD of 4 determinations) are expressed as % input. H1975/CshRNA and H1975/STAT1shRNA (J) or H1975/STAT2shRNA (K) cells treated with 1 μM OSI for 3 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for CshRNA cells (assigned a value of 1).

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