Fig. 3: MUC1-C→type I IFN pathway regulates A3A expression. | Cell Death Discovery

Fig. 3: MUC1-C→type I IFN pathway regulates A3A expression.

From: Activation of APOBEC3 cytidine deaminases and endogenous retroviruses is integrated by MUC1-C in NSCLC cells

Fig. 3: MUC1-C→type I IFN pathway regulates A3A expression.The alternative text for this image may have been generated using AI.

A MGH170 cells treated with 1 μM OSI or 1 μM SAV for 3 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for control cells (assigned a value of 1). B MGH170 cells treated with 1 μM OSI + 1 μM SAV for 3 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for control cells (assigned a value of 1). C Chromatin from MGH170 cells treated with 1 μM OSI + 1 μM SAV was immunoblotted with antibodies against the indicated proteins. D, E MGH170 cells expressing the designated vectors were treated with OSI + SAV for 3 days and vehicle or DOX for 7 days and analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1) (D). Lysates were immunoblotted with antibodies against the indicated proteins (E). F Soluble chromatin from MGH170 cells treated with OSI + SAV was precipitated with antibodies against MUC1-C, STAT1, STAT2 and IRF9. The DNA samples were amplified by qPCR with primers for the A3A dELS1 region. The results (mean ± SD of 3 determinations) are expressed as % input. G Chromatin from MGH170 cells treated with OSI + SAV was analyzed for accessibility of the A3A dELS-1 region by nuclease digestion. The results (mean ± SD of 3 determinations) are expressed as % undigested chromatin. MGH170/CshRNA and MGH170/STAT1shRNA (H) or MGH170/STAT2shRNA (I) cells treated with OSI + SAV were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for CshRNA cells (assigned a value of 1). J Schema depicting the MUC1-C→U-ISGF3 auto-inductive pathway that regulates A3A expression.

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