Fig. 4: MUC1-C→type I IFN signaling regulates A3G and other A3s. | Cell Death Discovery

Fig. 4: MUC1-C→type I IFN signaling regulates A3G and other A3s.

From: Activation of APOBEC3 cytidine deaminases and endogenous retroviruses is integrated by MUC1-C in NSCLC cells

Fig. 4: MUC1-C→type I IFN signaling regulates A3G and other A3s.The alternative text for this image may have been generated using AI.

GSEA of RNA-seq data from H1975-OR vs H1975 cells (A) and for H1975-OR/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days (B) using the REACTOME INTERFERON SIGNALING gene signature. C Analysis of RNA-seq data from H1975 and H1975-OR cells for levels of A3 transcripts. D Analysis of RNA-seq data from H1975-OR/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days for levels of A3 transcripts. The results are expressed as the mean ± SD of 3 biologically independent samples. E Lysates from H1975/tet-MUC1shRNA cells treated with 1 μM OSI for 3 days and vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. F H1975/tet-MUC1shRNA cells treated with 1 μM OSI for 3 days and vehicle or DOX for 7 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for control cells (assigned a value of 1). H1975/CshRNA, H1975/STAT1shRNA (G) and H1975/STAT2shRNA (H) cells treated with 1 μM OSI for 3 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for CshRNA cells (assigned a value of 1). I MGH170 cells treated with OSI + SAV were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for control cells (assigned a value of 1). J MGH170/tet-MUC1shRNA treated with OSI + SAV for 3 days and vehicle or DOX for 7 days were analyzed for the indicated transcripts by qRT-PCR. The results (mean ± SD of 4 determinations) are expressed as relative levels compared to that obtained for vehicle-treated cells (assigned a value of 1). K Lysates from MGH170/tet-MUC1shRNA cells treated with OSI + SAV for 3 days and vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. L Schema of A3G with highlighting of the pELS region containing a U-ISGF3 binding motif. Soluble chromatin from MGH170/tet-MUC1shRNA cells treated with OSI + SAV for 2 days and vehicle or DOX was precipitated with antibodies against MUC1-C, STAT1 and STAT2. The DNA samples were amplified by qPCR with primers for the A3G pELS region. The results (mean ± SD of 4 determinations) are expressed as % input.

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