Fig. 1: Virtual screening for MTAs identified compound 89 as a new potential anti-tumor small molecule.

A High-throughput virtual screening workflow for new tubulin inhibitors. B HeLa and HCT116 cells were treated with the indicated compounds, and MTS assay was performed after 24 h. C The chemical structures of compounds 82 and 89, and their IC50 values against HeLa and HCT116 cells.