Fig. 3: Compound 89 induced cycle arrest and apoptosis in tumor cells. | Cell Death Discovery

Fig. 3: Compound 89 induced cycle arrest and apoptosis in tumor cells.

From: Discovery of a novel potent tubulin inhibitor through virtual screening and target validation for cancer chemotherapy

Fig. 3

A HeLa and HCT116 cells were treated with indicated concentrations of 89 and co-incubated for 24 h. Cell population distribution was determined following PI staining and further analyzed by flow cytometry. Bars represent mean ± SD of three independent experiments. B HeLa, HCT116 and 4T1 cells were treated with indicated concentrations of 89 and the expression of cell cycle-related proteins was detected by Western immunoblotting assay with the indicated antibodies. C HeLa cells were treated with 89 at the indicated doses. Nuclei were stained with DAPI (blue) by immunofluorescence staining. Scale bar, 20 μm. D HeLa, HCT116 and 4T1 cells were left untreated or treated with 89 at the indicated doses for 24 h. Apoptotic cells were labeled with annexin V and PI and analyzed by flow cytometry. Bars represent mean ± SD of three independent experiments. E HeLa, HCT116 and 4T1 cells were treated with indicated concentrations of 89 and the expression of cleaved-PARP, Bax and Bcl-2 were detected by Western immunoblotting assay with the indicated antibodies. Protein expressions were quantitated by densitometry and normalized against that of actin. Bars represent mean ± SD of three independent experiments.

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