Fig. 5: Compound 89 suppressed tumor cell proliferation via disrupting microtubule network and organization. | Cell Death Discovery

Fig. 5: Compound 89 suppressed tumor cell proliferation via disrupting microtubule network and organization.

From: Discovery of a novel potent tubulin inhibitor through virtual screening and target validation for cancer chemotherapy

Fig. 5

A, B HeLa cells were treated with increasing concentrations of 89 for 24 h. Tubulin was stained with anti-α-tubulin (green) and anti-β-tubulin (red) antibodies, while the nucleus (blue) was stained with DAPI. The boxed areas were magnified to show clearer changes of the microtubule network in 89-treated cells by immunofluorescence assay. C HeLa, HCT116 and 4T1 cells were treated with indicated concentrations of 89 and the expressions of α and β-tubulin were detected by Western immunoblotting assay with the indicated antibodies. Protein expressions were quantitated by densitometry and normalized against that of actin. D HCT116 cells were treated with increasing concentrations of 89, and then soft agar colony formation assay was performed. Bars represent mean ± SD of three independent experiments. ns, no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group.

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