Fig. 1: Increasing lobeline concentrations leads to a corresponding increase in retained SGs post-hypoxia. | Cell Death Discovery

Fig. 1: Increasing lobeline concentrations leads to a corresponding increase in retained SGs post-hypoxia.

From: Modulation of stress granules by lobeline increases cell death in hypoxia and impacts the ability of glioblastoma cells to secrete extracellular vesicles

Fig. 1

A, B Human immortalized GBM U251 cells were treated with increasing concentrations of lobeline (0–100 μM) for 1 h prior to a 2 h incubation ± hypoxia (<1% O2). Cells were allowed to recover for 30 min before being fixed and stained for cellular membranes (WGA), SGs (TIAR and G3BP2) and nuclei (DAPI). Images were processed through a CellProfiler automated pipeline for quantification of A percent cells with SGs and B average number of SGs per cell (in those cells with SGs) based on correlative TIAR and G3BP2 immunostaining. Data are presented as the mean of biological replicates (N = 3) ± SEM, multiple unpaired t-tests *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. C Representative immunofluorescence of U251 cells (from A, B) treated with 0, 50 and 100 μM lobeline ± hypoxia.

Back to article page