Fig. 6: Lobeline dampens hypoxia-induced EV release and sequesters YBX1 in SGs. | Cell Death Discovery

Fig. 6: Lobeline dampens hypoxia-induced EV release and sequesters YBX1 in SGs.

From: Modulation of stress granules by lobeline increases cell death in hypoxia and impacts the ability of glioblastoma cells to secrete extracellular vesicles

Fig. 6

A U251 cells were treated with 50 μM lobeline or vehicle control for 1 h prior to a 2 h incubation ± hypoxia (<1% O2). EVs were isolated from conditioned culture media harvested from U251 cells 2 h post hypoxic or corresponding normoxic incubation and quantified by nanoparticle tracking analysis. The average of each biological replicate (N = 3) is expressed as a fold change of particles/mL of media ± SEM, one-way ANOVA, Tukey’s multiple comparisons test *p < 0.05, **p < 0.01. B Representative immunofluorescence of U251 cells. U251 cells were treated as above, were fixed and stained for actin (phalloidin), SGs (TIAR), YBX1 and nuclei (DAPI) at T = 60 min post-hypoxia.

Back to article page