Fig. 1: Identifying PLSCR1 as a potential target of NEDD4-2.
From: Phospholipid scramblase 1 (PLSCR1) is a novel substrate of NEDD4-2 (NEDD4L) mediated ubiquitination
A Proteomic enrichment analysis shows phospholipid scramblase 1 (PLSCR1) as the most enriched protein in the pulldown enrichment dataset (Adj. p-value of 0.03) after NEDD4-2, both highlighted in red. B Sequence alignment (CLUSTAL-Omega (EMBL-EBI)) of PLSCR1 for human and mouse homologues: Homo sapiens PLSCR1 (O15162) and Mus musculus PLSCR1 (Q9JJ00). Protein domains that suggest features of NEDD4-2 interacting proteins are highlighted and the PPxY motifs are underlined. Immunoblotting showing increased PLSCR1 protein level in C NEDD4-2 KO CCD kidney cells (N42 KO), D NEDD4-2 siRNA treated HEK293T cells (siRNA1, 2) and (E) after the inhibition of proteasomal and lysosomal degradation by MG132 and chloroquine (CQ) treatment respectively in CCD cells compared to untreated controls (CT); n = 3 ± SD, significance was determined using unpaired two-tailed Student’s t-test, *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001.
