Fig. 1: CRISPR library screening identified SUV39H2 as a driver for oHSV-1 resistance.

A A schematic diagram illustrates the workflow of genome-wide CRISPR/Cas9 knockout library screening. The human genome-wide CRISPR/Cas9 knockout libraries A and B were packaged into lentiviral particles and transduced into SCC15 cells at an MOI of 0.3. The sgRNA-transduced cells were selected with puromycin to generate a mutant cell pool. Mutant cells were then cultured with oHSV-1 for 48 and 72 h for genetic screening. Genomic DNA was extracted from the treated cells, and the sgRNA fragments were amplified by PCR. The copy number of sgRNAs was determined by high-throughput sequencing and analyzed using the MAGeCK v0.5.7 algorithm. B A Venn diagram illustrates the overlap of genes identified in the genome-wide CRISPR/Cas9 knockout library screening between SCC15 cells infected with oHSV-1 and WISH cells infected with HSV-1. C GO analysis was performed on the overlap of genes. D The rankings of SUV39H2 and MAVS in the negative screening are indicated by the black line. E The sgRNAs targeting SUV39H2 were consistently depleted in Treatment group.