Fig. 5: SUV39H2 inhibits oHSV-1 gene transcription by binding to the viral promoter. | Cell Death Discovery

Fig. 5: SUV39H2 inhibits oHSV-1 gene transcription by binding to the viral promoter.

From: CRISPR/Cas9 screening identifies SUV39H2 as a key regulator of oHSV-1 resistance in oral squamous cell carcinoma

Fig. 5

A–C SCC15 cells with either SUV39H2 knockdown or overexpression, or those treated with OTS186935, were infected with oHSV-1 at an MOI of 1, and samples were collected at various time points post-infection. The transcriptional levels of IE (ICP0), E (ICP8), and L (gD) viral genes were quantified by qRT-PCR. D HEK293T cells were co-transfected with VP16 (25 ng) and ICP0-Luc (10 ng), combined with 3HA-SUV39H2 or empty vector. At the same time, pCMV-β-gal (50 ng) was transfected to normalize the transfection efficiency. At 24 h post-transfection, luciferase activities were measured and corrected by b-gal catalytic activities. E SUV39H2 knockdown SCC15 cells and NC cells infected with oHSV-1 (MOI = 3) for 3 h. The ChIP assay shown the levels of H3K9me3 on viral promoters. F SCC15 cells treated with 0.5 μM OTS186935 for 24 h and then infected with oHSV-1 (MOI = 3) for 3 h. The ChIP assay shown the levels of H3K9me3 on viral promoters. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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