Fig. 4: Trajectory analysis identifies positive correlation between CD44 expression and 1C metabolism in diffuse-like intestinal-type gastric cancer. | Cell Death Discovery

Fig. 4: Trajectory analysis identifies positive correlation between CD44 expression and 1C metabolism in diffuse-like intestinal-type gastric cancer.

From: One-carbon metabolic pathway is a novel molecular signature for CD44-positive intestinal-type gastric cancer

Fig. 4

A UMAP plot showing nine individual clusters. B UMAP plots displaying ‘CD44-high’ and ‘CD44-low’ clusters of GC141, alongside the clonal samples of GC143. C Dimplot visualizing CD44 expression. D Vlnplot showing expression of CD44 within each clusters. E, F Prediction of trajectories using ‘Slingshot’ analysis; ‘Slingshot’ results illustrating the two individual trajectories of lineages as lines, with arrows indicating the direction of pseudotime progression. G Scatterplots depicting the positive correlation levels of 1C metabolism-related genes along the pseudotime 1 axis. Dots: single cells; colors: clusters. (*R > 0.1). H UMAP plot of trajectory analysis performed using ‘Monocle3.’ I Scatterplots representing the positive correlation of 1C metabolism-related gene expression with pseudotime in ‘Monocle3’ trajectory analysis.

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