Fig. 5: METTL3 overexpression disrupted nasal epithelial cell adhesion and induced macrophage M1 polarization through enhancing circCDKAL1 expression. | Cell Death Discovery

Fig. 5: METTL3 overexpression disrupted nasal epithelial cell adhesion and induced macrophage M1 polarization through enhancing circCDKAL1 expression.

From: METTL3-mediated m6A modification of circCDKAL1 regulates macrophage M1 polarization and nasal epithelial cell barrier function in allergic rhinitis through IGF2BP2/JARID2/HMGB1 axis

Fig. 5: METTL3 overexpression disrupted nasal epithelial cell adhesion and induced macrophage M1 polarization through enhancing circCDKAL1 expression.

HNEpCs were transfected with OE-METTL3 or together with sh-circCDKAL1 and followed by OVA treatment. n = 4. A TER was examined using EVOM/EndOhm system. n = 4. B FD4 levels in mice were evaluated using CLARIOstar. n = 4. C, D ZO-1, Occludin and Claudin-1 expression was detected using western blot and IF assay. n = 4. HNEpCs were transfected with OE-METTL3 or together with sh-circCDKAL1 and followed by OVA treatment, which was co-cultured with MH-S cells for 24 h. E The expression of IL-6, TNF-α, Arg-1and IL-10 in MH-S cells were examined by RT-qPCR. n = 4. F CD86+ and CD206+ positive macrophages were detected using flow cytometry. n = 4. *P < 0.05, **P < 0.01.

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