Fig. 6: SLC25A10 affects the resistance of cervical cancer cells to ferroptosis by reducing GSH and GPX4.

Quantitative studies of the GSH content in HeLa cells revealed that the GSH content decreased after SLC25A10 knockdown, whereas the GSH content increased in SLC25A10-overexpressing cells (A). Quantitative studies on the MDA content in HeLa cells treated with erastin or DDP revealed that the MDA content increased more in cells with low SLC25A10 expression than in control cells; in contrast, the MDA content did not increase significantly in the group with high SLC25A10 expression, and the difference was not statistically significant. DMSO or NS was used as a control (B, C). Quantitative analyses of ROS levels in HeLa cells treated with erastin or DDP, with or without Ferr-1. Under erastin or DDP stimulation, the ROS levels in the knockdown group were significantly greater than those in the control group, whereas those in the overexpression group were relatively lower. This phenomenon was reversed by Ferr-1. DMSO or NS was used as a control (D–G). Quantitative studies on the changes in Fe²⁺ levels in HeLa cell lines treated with erastin or DDP, with or without Ferr-1. Under stimulation with erastin or DDP, the knockdown group exhibited significantly higher Fe²⁺ levels than the control group, while the overexpression group had relatively lower levels. This phenomenon was reversed by Ferr-1. DMSO or NS was used as a control (H–K). Additionally, we investigated the effect of cisplatin on the expression of GPX4, ACSL4, and SLC7A11 in HeLa cell lines by detecting protein expression levels and quantifying the results (L–N). HeLa control cells or SLC25A10-overexpressing cells were treated with 10 µM erastin for 24 h and then prepared for transmission electron microscopy. The control group exhibited typical mitochondrial changes associated with ferroptosis, that is, a reduced or absent mitochondrial ridge, increased membrane density, and increased permeability. The morphological changes in the mitochondria of the SLC25A10-overexpressing cells were less substantial (O). Compared with the control group, ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 3. Scale =100 µm, 50 µm and 1 µm. The data represent the means ± SDs.