Fig. 5: HIF-1α was identified as a potential target of miR-122-5p.

A The Venn diagram shows the results of three different prediction tools (PITA, miRanda, and RNAhybrid) that were used to predict the target genes of miR-122-5p; B A miRNA-mRNA target gene network diagram is shown, featuring the relationship between miR-122-5p and its predicted target gene (mRNA); C Enrichment analysis was performed on the genes predicted by miR-122-5p; D Predicted potential miR-122-5p binding site in the 3′-UTR of HIF-1α mRNA; E Luciferase activity in NRK-49F cells transfected with negative control or miR-122-5p mimic together with a reporter vector containing HIF-1α-mut binding sequences. Results are shown as mean ± SD for three individual experiments. **P < 0.01.