Fig. 8: The overexpression of HIF-1α reversed the ameliorative effect of MiR-122-5p mimic on a TGF-β1 exosome-induced NRK-49F cell model of fibrosis. | Cell Death Discovery

Fig. 8: The overexpression of HIF-1α reversed the ameliorative effect of MiR-122-5p mimic on a TGF-β1 exosome-induced NRK-49F cell model of fibrosis.

From: Exosomal miR-122-5p from tubular cells ameliorates renal interstitial fibrosis by regulating fibroblasts via HIF-1α

Fig. 8

A The expression levels of α-SMA, HIF-1α, Phospho-SMAD2 (Ser465/Ser467), Smad2, Phospho-SMAD3 (Ser423/425), Smad3, E-cadherin, and Smad7 in NRK-49F cells were detected by western blotting. B Relative expression levels ofα-SMA; C Relative expression levels of HIF-1α; D Relative expression levels of Smad2; E Relative expression levels of Phospho-SMAD2 (Ser465/Ser467); F Relative expression levels of Smad3; G Relative expression levels of Phospho-SMAD3(Ser423/425); H Relative expression levels of Smad7; I The expression levels of Fibronectin in NRK-49F cells were detected by immunofluorescence (Bar = 50 μm); J Statistics of fibronectin expression levels in NRK-49F cells; K The expression levels of Col-I in NRK-49F cells were detected by immunofluorescence (Bar = 50 μm); L Statistics of Col-I expression levels in NRK-49F cells. Results are shown as mean ± SD for three individual experiments. **P < 0.01.

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