Fig. 4: FHL2 facilitates ITGB1-mediated ECM remodeling and activates FAK/MAPK signaling.
A Volcano plot showing differentially expressed genes between H1299-FHL2OE and parental H1299 cells based on transcriptome sequencing. B Gene Ontology (GO) enrichment analysis of differentially expressed genes in parental H1299 and H1299-FHL2OE cells. C, D Representative images and quantitative analysis of Comet assays after overexpression of FHL2 in A549 and H1299 cells, and knockdown of FHL2 in A549R and H1299R cells (n = 50, cell count). E–G Western blotting and quantitative analysis of the expression of FHL2 and key proteins in the FAK/MAPK pathway after overexpression of FHL2 in A549 and H1299 cells, and knockdown of FHL2 in A549R and H1299R cells under 0-Gy and 8 Gy irradiation conditions (n = 3). H Representative images of Immunofluorescence showing the phosphorylation of FAK in A549 and H1299 cells with FHL2 overexpression, and in A549R and H1299R cells with FHL2 knockdown. I Clonogenic assay demonstrating the FAK inhibitor PF-562271 decreased survival rates of irradiated A549 and H1299 cells including those overexpressing FHL2 (n = 3). J, K Western blotting and quantitative analysis of FHL2 and key proteins in the FAK/MAPK pathway after FHL2 overexpression and PF-562271 treatment in A549 and H1299 cells (n = 3). Mean ± SD. *p < 0.05, **p < 0.01.
