Fig. 3: Transcriptomic sequencing reveals LINC01198 positively regulates cytokine IL-1β expression. | Cell Death Discovery

Fig. 3: Transcriptomic sequencing reveals LINC01198 positively regulates cytokine IL-1β expression.

From: LINC01198 activates Hippo signaling to stimulate IL-1β autocrine for driving vemurafenib resistance by associating with TAOK1/2 in melanoma

Fig. 3: Transcriptomic sequencing reveals LINC01198 positively regulates cytokine IL-1β expression.

A Venn analysis was performed to find common upregulated factors from three RNA-Seq comparable pairs: A375P VS. A375R, A375P with mock treatment VS. A375P treated with 2 μM VEM, wild type LINC01198 VS. LINC01198_KO strains. B Gene Ontology (GO) analysis of the differentially-expressed genes in A375R cells. Cytokine related signaling pathways are highlighted. C The heatmap of differentially expressed LncRNAs between A375R cells and A375R with LINC01198 knockout. D Differentially expressed genes between A375R cells and A375R KO_LINC01198 were determined by RNA-Seq and shown by volcano plot. LINC01198 and IL1B were shown by lead marks. The expression levels of LINC01198 and IL1B in melanoma cells were determined by qPCR after LINC01198 knockout E, knockdown F and LINC01198 overexpression G. H The expression levels of IL1B in A375P and A375R cells were detected by qPCR. I Levels of mature IL-1β in culture medium of A375P and A375R cells were measured by ELISA assay. J The expression levels of IL1R1 and IL1RL1 in A375P and A375R cells were detected by qPCR. Data are presented as means ± SD, and p values are determined using two-tailed Student’s t-test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Back to article page