Fig. 1: Conditioned medium (CM) of cancer cells promoted osteoclastic pre-metastatic niche formation. | Cell Death Discovery

Fig. 1: Conditioned medium (CM) of cancer cells promoted osteoclastic pre-metastatic niche formation.

From: Therapeutic targeting of STING-IL6/STAT3 axis to inhibit osteoclastic niche formation and breast cancer bone metastasis

Fig. 1: Conditioned medium (CM) of cancer cells promoted osteoclastic pre-metastatic niche formation.

A Experimental design for the collection and injection of CM. B–I TRAP staining (from top to bottom: femur, tibia, spine, and skull). Scale bars, 1000 µm. On the right is a quantitative analysis of the number of TRAP+ cells per bone marrow area. n = 6 per group. J–Q Representative images of immunofluorescence staining of CTSK (red) and Netrin-1 (green) in the femur, tibia, spine, and skull (left, from top to bottom) and quantitative analysis (right). Scale bars, 1000 µm and 100 µm. n = 6 per group. Data are presented as mean ± SD. Significance levels are indicated on top of each comparison. Comparisons between the two groups were performed using an unpaired two-sided Student’s t-test.

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