Fig. 2: More potent tau hyperphosphorylation in APOE4/4 carried AS mice model.
From: APOE4 promotes nigral tau hyperphosphorylation through cholesterol in atherosclerosis
A Representative HE staining of coronary arteries. Bar, 20 μm. B Lesion degree of coronary arteries in AS mice. Black dashed line showed that area of plaque. n = 6, two-way ANOVA followed by Bonferroni’s post hoc tests. Bar, 100 μm. Treatment: F(1, 20) = 129.4, p < 0.001, Genotype: F(1, 20) = 40.9, p < 0.001, Phenotype × Genotype: F(1, 20) = 40.9, p < 0.001. C Representative IF staining of TH, AT8 and pSer396 tau in mice SNpc. Bar, D Relative intensity of AT8 and pSer396 tau in TH positive cells. n = 6, two-way ANOVA followed by Bonferroni’s post hoc tests. AT8, Treatment: F(1, 20) = 247.7, p < 0.001, Genotype: F(1, 20) = 19.17, p < 0.001, Phenotype × Genotype: F(1, 20) = 12.16, p < 0.001. pSer396, Treatment: F(1, 20) = 217.2, p < 0.001, Genotype: F(1, 20) = 31.13, p < 0.001, Phenotype × Genotype: F(1, 20) = 32.19, p < 0.001. E, F Representative blots and quantification of phosphorylated tau at multiple sites and pSer129 α-syn in SNpc of postmortem brain tissues. two-way ANOVA followed by Bonferroni’s post hoc tests. pS129 α-syn, Treatment: F(1, 20) = 402.7, p < 0.001, Genotype: F(1, 20) = 114.1, p < 0.001, Phenotype × Genotype: F(1, 20) = 109.8, p < 0.001, AT8, Treatment: F(1, 20) = 247.7, p < 0.001, Genotype: F(1, 20) = 19.17, p < 0.001, Treatment × Genotype: F(1, 20) = 12.16, p = 0.002, pS396 tau, Treatment: F(1, 20) = 216.6, p < 0.001, Genotype: F(1, 20) = 25.49, p < 0.001, Treatment × Genotype: F(1, 20) = 20.25, p < 0.001, pS202 tau, Treatment: F(1, 20) = 126.5, p < 0.001, Genotype: F(1, 20) = 25.43, p < 0.001, Treatment × Genotype: F(1, 20) = 29.25, p < 0.001, pS404 tau, Treatment: F(1, 20) = 204.7, p < 0.001, Genotype: F(1, 20) = 58.46, p < 0.001, Treatment × Genotype: F(1, 20) = 52.68, p < 0.001, pT231 tau, Treatment: F(1, 20) = 208.1, p < 0.001, Genotype: F(1, 20) = 26.87, p < 0.001, Treatment × Genotype: F(1, 20) = 19.85, p < 0.001, pT205, Treatment: F(1, 20) = 195.7, p < 0.001, Genotype: F(1, 20) = 22.79, p < 0.001, Treatment × Genotype: F(1, 20) = 15.40, p < 0.001, Pt217, Treatment: F(1, 20) = 131.4, p < 0.001, Genotype: F(1, 20) = 28.59, p < 0.001, Phenotype × Genotype: F(1, 20) = 35.64, p < 0.001, pT214, Treatment: F(1, 20) = 61.87, p < 0.001, Genotype: F(1, 20) = 6.472, p = 0.019, Treatment × Genotype: F(1, 20) = 2.122, p = 0.160, pT181, Treatment: F(1, 20) = 68.50, p < 0.001, Genotype: F(1, 20) = 14.23, p = 0.001, Treatment × Genotype: F(1, 20) = 8.559, p = 0.008, total tau, Treatment: F(1, 20) = 0.022, p = 0.882, Genotype: F(1, 20) = 0.097, p = 0.758, Treatment × Genotype: F(1, 20) = 1.104, p = 0.306. *p < 0.05, **p < 0.01, and ***p < 0.001.
