Fig. 2: CREB5 drives lymph node metastasis of CCa in vivo.
From: CREB5 promotes nodal metastasis of cervical cancer by regulation of APLN-induced lymphangiogenesis
A, B Schematic illustration of the in vivo lymphatic metastasis model using nude mice. CCa cells stably expressing control (shGFP) or CREB5-targeting shRNA (shCREB5) were injected into the footpad of mice to assess popliteal LN metastasis. C, D Representative images of popliteal LNs harvested from shGFP and shCREB5 groups (n = 6 per group). E, F Immunostaining for pan-cytokeratin (a marker for epithelial cancer cells) in popliteal LNs, confirming metastatic CCa cells. G, H Quantitative analysis of popliteal LN volumes in the shGFP and shCREB5 groups. I, J Metastatic area within popliteal LNs.
