Fig. 4: Analysis of fusion, fission, and mitophagy in the absence of Ezrin. | Cell Death Discovery

Fig. 4: Analysis of fusion, fission, and mitophagy in the absence of Ezrin.

From: Loss of Ezrin triggers mitochondrial dysfunction and oxidative stress, associated with neuronal cell death

Fig. 4

A WT and EZR−/− HeLa cells were lysed, and proteins were incubated with anti-TOM20, anti-OPA1, anti-MFN2, anti-MFN1, anti-DRP1, anti-PINK1, and anti-FIS1 antibodies. GAPDH was used as a loading control. The graphs show the levels of OPA1, MFN2, MFN1, DRP1, PINK1, pPARKIN, PARKIN, FIS1, and TOM20 relative to GAPDH ± SEM (at least n = 3 experiments). Statistical test: unpaired t-test. B Immunofluorescence analysis of WT and EZR−/− HeLa cells stained with LAMP1 (red), TOM20 (green), and DAPI (blue) antibodies reveals increased mitophagy, as indicated by the colocalization of TOM20 and LAMP1 signals in the absence of EZRIN.

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