Fig. 6: Fosinopril induced pyroptosis and antitumor activity by activating ROS through MOMP in A549 cell xenografts in vivo.
From: Fosinopril mediates antitumor efficacy by inducing GSDME-dependent pyroptosis in NSCLC
A Treatment schedule for mice with A549 xenograft tumors and fosinopril treatment. B–E Effect of fosinopril on tumor weight, volume weight, and body weight in A549 xenograft model (n = 5 per group). F Hematoxylin and eosin (HE) staining of tumor tissues (HE, original magnification, ×200; Scale bar = 50 µm). G Tumor cells co-stained with PI and Annexin V were visualized by immunofluorescence assays. Quantitative analysis of fluorescence intensity is shown. H Immunofluorescence analysis of GSDME localization in tumor cells. Quantitative analysis of fluorescence intensity is shown. I Protein expression levels of Bax, Cleaved-Caspase-3, Cleaved-Caspase-9, and GSDME-N in tumor tissues were detected by Western blot. J ROS levels in tumor cells were detected by immunofluorescence assays (n ≥ 3, ***P < 0.001 vs. fosinopril 0 mg/kg group).
