Fig. 2: Tan IIA alleviates the heat-induced malignancy enhancement of HCC cells. | Cell Death Discovery

Fig. 2: Tan IIA alleviates the heat-induced malignancy enhancement of HCC cells.

From: Tanshinone IIA inhibits heat-induced growth of p53-mutant Huh-7 hepatocellular carcinoma by modulating osmotic homeostasis and glycolysis through targeting ALDH7A1

Fig. 2: Tan IIA alleviates the heat-induced malignancy enhancement of HCC cells.

A, B Heat pretreatment at 44 °C enhances the invasion of Huh-7 cells, and Tan IIA inhibits the invasion with or without heat pretreatment. C, D Heat pretreatment induces apoptosis of Huh-7 cells, and Tan IIA increases the apoptosis of the cells with or without the heat pretreatment. E, F Heat pretreatment increases the percentage of HCC cells in the G1 phase, and Tan IIA increases the cells in the G2/M phase, and decreases those in the G1 phase. G, H Heat pretreatment increases the protein level of the markers for cell invasion and/or EMT, MMP2 and N-cadherin, and Tan IIA decreases the protein levels of MMP-2/9 and N-cadherin. I, J Heat pretreatment increases the protein level of the anti-apoptotic marker Bcl2 and the G1-S transition markers cyclin D1 and CDK1, and decreases the apoptotic markers Bax and cleaved Caspase 3, whereas Tan IIA restores these effects. *p < 0.05, **p < 0.01, compared with NC at 37 °C; # p < 0.05, ## p < 0.05, compared with NC at 44 °C.

Back to article page