Fig. 3: Bioinformatics after mRNA sequencing for Huh-7 cells indicate that oxidoreductase ALDH7A1 in amino acid metabolic pathways may play important roles in anti-HCC effects of Tan IIA after heat stress induction. | Cell Death Discovery

Fig. 3: Bioinformatics after mRNA sequencing for Huh-7 cells indicate that oxidoreductase ALDH7A1 in amino acid metabolic pathways may play important roles in anti-HCC effects of Tan IIA after heat stress induction.

From: Tanshinone IIA inhibits heat-induced growth of p53-mutant Huh-7 hepatocellular carcinoma by modulating osmotic homeostasis and glycolysis through targeting ALDH7A1

Fig. 3

KEGG pathway enrichment of DEGs between the NC and the Tan IIA groups (A), as well as the Heat and Heat + Tan IIA groups (B). The blue dashed boxes represent the pathways that are shared between the two comparisons, while the green dashed boxes represent pathways that are different from each other. C GSEA was conducted on 4 KEGG pathways (Cell cycle, Arginine and proline metabolism, Serine and threonine metabolism, and Pyruvate metabolism) in the comparison between the Heat and the Heat + Tan IIA groups. D GO enrichment of the DEGs between the Heat and the Heat + Tan IIA groups. The blue solid box indicates the most significantly enriched GO term. E The Venn diagram illustrates the overlaps among the three gene sets, which were significantly enriched in the two KEGG pathway terms (Arginine and proline metabolism, and Glycine, serine and threonine metabolism) and one GO term (Oxidoreductase activity). F–H The relative expression level (data extracted from the RNA-seq results) of the 3 chosen genes MAOB, DAO, and ALDH7A1 in heat-induced Huh-7 cells treated with Tan IIA. **p < 0.01, compared with NC at 37 °C; # p < 0.05, ## p < 0.05, compared with NC at 44 °C; NS not significant.

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