Fig. 4: Tan IIA reduces ALDH7A1 and intracellular osmolytes and inhibits glycolysis in the heat stress-induced HCC cells. | Cell Death Discovery

Fig. 4: Tan IIA reduces ALDH7A1 and intracellular osmolytes and inhibits glycolysis in the heat stress-induced HCC cells.

From: Tanshinone IIA inhibits heat-induced growth of p53-mutant Huh-7 hepatocellular carcinoma by modulating osmotic homeostasis and glycolysis through targeting ALDH7A1

Fig. 4

A–C The altered mRNA level of MAOB, DAO, and ALDH7A in Huh-7 cells with or without heat pretreatment validated using qPCR. D–G The altered protein level of MAOB, DAO and ALDH7A in Huh-7 cells with or without heat pretreatment validated using western blotting. H–J Heat pretreatment induces increased glucose uptake and ATP production, and decreased the production of lactate, and Tan IIA reduces these indices to various extents. K–S Seahorse analyses of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR): heat-induced cells have higher OCR and ECAR values, and Tan IIA can enhance basal respiration and lower some ECAR values. T Tan IIA treatment results in the decrease in NADH production with or without heat pretreatment. U, V Tan IIA reduces the osmolytic and enzymatic products betaine and d-glycerate to various extents with or without heat pretreatment. W–Z Heat pretreatment and Tan IIA treatment induce ROS and inhibit LPO to various extents. *p < 0.05, **p < 0.01, compared with NC at 37 °C; # p < 0.05, ## p < 0.05, compared with NC at 44 °C; NS not significant.

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