Fig. 1: Elevated DEPTOR protein expression associated with decreased mTORC1, mTORC2 signaling and System A and System L amino acid transport activity in FGR PHT cells. | Cell Death Discovery

Fig. 1: Elevated DEPTOR protein expression associated with decreased mTORC1, mTORC2 signaling and System A and System L amino acid transport activity in FGR PHT cells.

From: Normalization of trophoblast mTOR signaling rescues impaired function in primary human trophoblast cells isolated from pregnancies complicated by fetal growth restriction

Fig. 1: Elevated DEPTOR protein expression associated with decreased mTORC1, mTORC2 signaling and System A and System L amino acid transport activity in FGR PHT cells.

Representative western blots for a DEPTOR, b S6 (Ser-235/236), c S6, d Akt (Ser-473) and e Akt in cell lysates of AGA and FGR PHT cells. Histogram summarizes the Western blotting data. Equal loading was performed. After normalization to total protein, the mean density of AGA samples was assigned an arbitrary value of 1. f, g System A activity was measured as the Na+-dependent uptake of [14C] MeAIB and System L activity was determined as BCH-inhibitable uptake of [3H] leucine. System A (f) and System L (g) transporters activity were lowered in FGR primary human trophoblast cells. Values are given as means  ± sem. *P < 0.05 vs. AGA, n = 5 each group, Student’s t test.

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