Fig. 2: Effect of DEPTOR siRNA silencing on DEPTOR protein expression in FGR PHT cells.

a Representative Western blot for DEPTOR in cell lysates of AGA and FGR PHT cells transfected with either scramble or DEPTOR siRNA. b Histogram summarizes the Western blotting data. Equal loading was performed. After normalization to total protein, the mean density of AGA samples was assigned an arbitrary value of 1. Values are given as means sem. *P < 0.05 vs. AGA PHT cells transfected with scramble siRNA; Means without a common letter differ significantly (P < 0.05) by One-way ANOVA with Tukey–Kramer multiple comparisons post hoc test.