Fig. 6: Normalizing DEPTOR protein level in FGR PHT cells restores Nedd 4-2 expression and prevents Nedd4-2 mediated ubiquitination of the SNAT2 and LAT1. | Cell Death Discovery

Fig. 6: Normalizing DEPTOR protein level in FGR PHT cells restores Nedd 4-2 expression and prevents Nedd4-2 mediated ubiquitination of the SNAT2 and LAT1.

From: Normalization of trophoblast mTOR signaling rescues impaired function in primary human trophoblast cells isolated from pregnancies complicated by fetal growth restriction

Fig. 6: Normalizing DEPTOR protein level in FGR PHT cells restores Nedd 4-2 expression and prevents Nedd4-2 mediated ubiquitination of the SNAT2 and LAT1.

a, b Representative Western blot for Nedd4-2 in cell lysates of AGA and FGR PHT cells transfected with either scramble or DEPTOR siRNA. c–f Decreased ubiquitination of SNAT2 and LAT1 in response to normalizing DEPTOR protein level in FGR PHT cells. Cell lysates from AGA and FGR PHT cells transfected with either scramble or DEPTOR siRNA were used for immunoprecipitation (IP) with an anti-SNAT2 (c, d) or LAT1 antibody (e, f). Immunoprecipitated proteins were separated by SDS-PAGE and blotted with an anti-ubiquitin (Ub) antibody. b, d, f Histogram summarizes the Western blotting data. Equal loading was performed. After normalization to total protein, the mean density of AGA samples was assigned an arbitrary value of 1. Values are given as means ±  sem. *P < 0.05 vs. AGA PHT cells transfected with scramble siRNA; Means without a common letter differ significantly (P  < 0.05) by One-way ANOVA with Tukey–Kramer multiple comparisons post hoc test.

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