Fig. 5: XMH95 differently from Hoechst 33258 triggers apoptosis and represses mitochondrial gene expression. | Cell Death Discovery

Fig. 5: XMH95 differently from Hoechst 33258 triggers apoptosis and represses mitochondrial gene expression.

From: Identification of a novel minor-groove DNA binder that represses mitochondrial gene expression and induces apoptosis in highly aggressive leiomyosarcoma cells

Fig. 5

A Cell death in SK-UT-1 cell line treated with the indicated concentrations of Hoechst 33258 or XMH95. Cell death was calculated 48 hours later as the percentage of cells positive to Trypan blue staining. Data are presented as mean + SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, relative to DMSO-treated cells (Untreated). B Venn diagram comparing the number of transcripts upregulated in SK-UT-1 cells after treatment for 6 or 18 hours with 10 µM of XMH95 or for 6 hours with 10 µM Hoechst 33258. C Bar plot of functional enrichments using the GSEA and the Molecular Signatures Database (MSigDB) tools. The analysis was performed for genes commonly upregulated by Hoechst 33258 and XMH95 in SK-UT-1 cells. Only the top terms are indicated. The detailed data are shown in Tables S10. D Bar plot of functional enrichments using the GSEA and the Molecular Signatures Database (MSigDB) tools. The analysis was performed for genes specifically upregulated by XMH95. Only the top terms are indicated. The detailed data are shown in Table S11. E Bar plot of functional enrichments using the GSEA and the Molecular Signatures Database (MSigDB) tools. The analysis was performed for genes specifically upregulated by Hoechst 33258. Only the top terms are indicated. The detailed data are shown in Table S12. F Venn diagram comparing the number of transcripts downregulated in SK-UT-1 cells after treatment for 6 or 18 hours with 10 µM of XMH95 or for 6 hours with 10 µM Hoechst 33258. G Bar plot of functional enrichments using the GSEA and the Molecular Signatures Database (MSigDB) tools. The analysis was performed for genes commonly downregulated by Hoechst 33258 and XMH95 in SK-UT-1 cells. Only the top terms are indicated. The detailed data are shown in Supplementary Table S13. H Bar plot of functional enrichments using the GSEA and the Molecular Signatures Database (MSigDB) tools. The analysis was performed for genes specifically downregulated by Hoechst 33258. Only the top terms are indicated. The detailed data are shown in Table S14. I Bar plot of functional enrichments using the GSEA and the Molecular Signatures Database (MSigDB) tools. The analysis was performed for genes specifically downregulated by XMH95. Only the top terms are indicated. The detailed data are shown in Table S15. J Regulation of mitochondrial gene expression in response to XMH95 or Hoechst treatments for the indicated times in HUtSMC and SK-UT-1 cells. Heatmap showing the expression levels (log2 fold change compared to untreated cells) of genes transcribed from the mitochondrial genome.

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