Fig. 6: Molecular analysis of migratory phenotype in acidosis induced subpopulations of MEL-JUSO melanoma cell lines.

a Relative SEMA3C mRNA expression (n = 3) of C₁₂FDG^high populations of MEL-JUSO CTR and MEL-JUSO LT NaHCO₃. Results are shown as mean ± SEM (range). Statistical analysis was made using Student’s unpaired t-test. b Relative CAV1 mRNA expression (n = 3) of C₁₂FDG^high populations of MEL-JUSO CTR and MEL-JUSO LT NaHCO₃. Results are shown as mean ± SEM (range). Statistical analysis was made using Student’s unpaired t-test. c Relative FLT1 mRNA expression (n = 3) of C₁₂FDG^high populations of MEL-JUSO CTR and MEL-JUSO LT NaHCO₃. Results are shown as mean ± SEM (range). Statistical analysis was made using Student’s unpaired t-test. d Relative S100B mRNA expression (n = 3) of C₁₂FDG^high populations of MEL-JUSO CTR and MEL-JUSO LT NaHCO₃. Results are shown as mean ± SEM (range). Statistical analysis was made using Student’s unpaired t-test. e Western blots of FLT1 and S100B protein expression of MEL-JUSO C₁₂FDG sorted subpopulations after LT acidosis treatment at pH 6.7 (LT NaHCO₃) compared to cells cultivated at pH 7.4 (CTR). Protein expression of β-actin was used as reference. Uncropped versions in Supplement Figs. 5 and 6. f Quantification of glycosylated FLT1 protein expression (n = 3) LT NaHCO₃ MEL-JUSO C₁₂FDG^high compared to LT NaHCO₃ MEL-JUSO C₁₂FDG^negative subpopulation. Relative protein level was normalized to actin. Results are shown as mean ± SEM (range). Statistical analysis was made using Student’s unpaired t-test. g Quantification of S100B protein expression (n = 3) of MEL-JUSO C₁₂FDG sorted subpopulations after LT acidosis treatment at pH 6.7 (LT NaHCO₃) compared to cells cultivated at pH 7.4 (CTR). Relative protein level was normalized to β-actin. Results are shown as mean ± SEM (range). Statistical analysis was made using two-way ANOVA. (*: p < .05, **: p < .01, ***: p < .001, ****: p < .0001).