Fig. 1: Single-cell transcriptomic analysis of the cellular composition in matched primary gastric tumors and metastatic lymph nodes. | Cell Death Discovery

Fig. 1: Single-cell transcriptomic analysis of the cellular composition in matched primary gastric tumors and metastatic lymph nodes.

From: Single-cell and spatial dissection of necroptosis spatiotemporal evolution driving lymph node metastasis in gastric cancer

Fig. 1: Single-cell transcriptomic analysis of the cellular composition in matched primary gastric tumors and metastatic lymph nodes.The alternative text for this image may have been generated using AI.

A Schematic diagram of the experimental workflow. We collected four primary tumor tissue samples (PT, n = 4) and two metastatic lymph node samples (MLN, n = 2) from four gastric cancer patients at Taizhou People’s Hospital affiliated with Nanjing Medical University. These samples were subjected to scRNA-seq and ST analysis using the 10x Genomics platform. B Single-cell UMAP plots for the six samples, with each plot displaying 23 cell clusters. Different colors represent different cell populations. C UMAP plot of 68 838 cells profiled in the present study colored by major cell types. D Bubble plots of the marker genes expressed in the major cell types. Dot color reflects expression level and dot size represents the percent of cells expressing marker genes in different cell types. E Expression of characteristic marker genes for the major cell types.

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