Fig. 3: Single-cell analysis of the metastatic mechanism of gastric cancer to lymph nodes. | Cell Death Discovery

Fig. 3: Single-cell analysis of the metastatic mechanism of gastric cancer to lymph nodes.

From: Single-cell and spatial dissection of necroptosis spatiotemporal evolution driving lymph node metastasis in gastric cancer

Fig. 3: Single-cell analysis of the metastatic mechanism of gastric cancer to lymph nodes.The alternative text for this image may have been generated using AI.

A High-resolution t-SNE analysis was performed to recluster epithelial cells, revealing 15 distinct cell clusters. Different colors represent different cell clusters. B A violin plot of copy number variation (CNV) levels in different epithelial cell clusters reflects the malignant characteristics of tumor cells. C The single-cell trajectory plots generated by Monocle [44] illustrate the reconstructed developmental pathways of tumor cells originating from PT and MLN. The trajectory features two primary branches: one is identified as the pathway of lymph node migration, while the other is deemed the pathway of primary tumor stay. D A volcano plot illustrates the differential gene expression across the different trajectories. E The heatmap shows the gene expression trend of each branch. The center of the heat map represents the start of the trajectory; moving from the center to the right represents differentiation from the start to cell fate 2 (LN metastasis branch), and moving from the center to the left represents differentiation from the root to cell fate 1 (PT branch). F Enrichment analysis results for the GCs clusters in the metastatic branch.

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