Fig. 1: Expression of DEPTOR in normal and degenerated human and rat intervertebral disks and expression of mTOR pathway–related proteins in senescent nucleus pulposus cells (NPCs). | Cell Death Discovery

Fig. 1: Expression of DEPTOR in normal and degenerated human and rat intervertebral disks and expression of mTOR pathway–related proteins in senescent nucleus pulposus cells (NPCs).

From: DEPTOR regulates nucleus pulposus cell senescence through the mTORC1/S6K1/ATG1 pathway to alleviate intervertebral disk degeneration

Fig. 1: Expression of DEPTOR in normal and degenerated human and rat intervertebral disks and expression of mTOR pathway–related proteins in senescent nucleus pulposus cells (NPCs).

A T2-weighted magnetic resonance images of normal (left) and degenerated (right) human disks. White arrows indicate degenerated disks. B Overview of donor nucleus pulposus (NP) tissue obtained during discectomy; scale bar: 1 cm. Hematoxylin and eosin (HE) and safranin O (SO) staining of degenerated human NP tissue from the intervertebral disk (right); scale bar: 200 µm. C Quantitative proteomics analysis via isobaric tags for relative and absolute quantitation (iTRAQ) showing DEPTOR protein levels in human intervertebral disk tissue. D Western blot (WB) detection and quantitative analysis of DEPTOR protein in human NP tissue. E DEPTOR detection in rat NP tissue; scale bar: 200 µm. F, G DOX-induced senescence in NPCs with WB detection and quantitative analysis of DEPTOR, mTORC1, S6K1, and ATG1. Data are presented as means ± standard deviations; ns nonsignificant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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