Fig. 3: Role of DEPTOR in aging-related proteins and extracellular matrix regulation in NPCs. | Cell Death Discovery

Fig. 3: Role of DEPTOR in aging-related proteins and extracellular matrix regulation in NPCs.

From: DEPTOR regulates nucleus pulposus cell senescence through the mTORC1/S6K1/ATG1 pathway to alleviate intervertebral disk degeneration

Fig. 3: Role of DEPTOR in aging-related proteins and extracellular matrix regulation in NPCs.

A WB detection and quantitative analysis of senescence markers P16, P21, and P53 in DOX-induced senescent NPCs. B, C Immunofluorescence and quantitative analysis of P16, P21, and P53 in DOX-induced NPCs; scale bar: 200 µm. D Reverse transcription–polymerase chain reaction (RT-PCR) analysis of P16, P21, and P53 expression in DOX-induced NPCs. E WB detection and quantitative analysis of extracellular matrix proteins (COL2 and ACAN) in DOX-induced NPCs. F, G Immunofluorescence and quantitative analysis of COL2 and ACAN in DOX-induced NPCs; scale bar: 200 µm. H RT-PCR analysis of COL2 and ACAN in DOX-induced NPCs. Results are presented as means ± standard deviations; ns nonsignificant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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