Fig. 1: Expression of ATXN3 in human cervical cancer tissue subtypes.

A ATXN3 mRNA expression levels in different human cervical cancer tissue subtypes. Tumor tissues analyzed: 21 HPV16⁺ SCCs (left) and 4 HPV18⁺ ACs (right). The qPCR data were analyzed using the comparative ΔΔCq method. The Cq values of the target gene were normalized to the endogenous control gene to calculate ΔCq values. For each matched pair of tumor (T) and adjacent non-tumor (NT) tissues, the ΔΔCq value was calculated. The relative gene expression change was expressed as log₂(fold change) = -ΔΔCq. The log₂(fold change) values from all samples were compared to the hypothetical value of 0 using a one-sample t test to determine statistical significance. The data are presented as the log₂(fold change) for individual samples. B Differential expression of ATXN3 protein in different human cervical cancer tissue subtypes. Tumor tissues analyzed: 17 HPV16⁺ SCCs (top) and 2 HPV18⁺ ACs (bottom). The statistical notations used in the figure are:ns P > 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Data are represented as mean ± SD. For comparisons involving the HPV18⁺ AC group (n = 2 or 4), the Mann–Whitney U test was used. All other comparisons between two groups were performed using a two-tailed unpaired Student’s t test.