Fig. 1: Ferroptosis occurs after SCIRI and HMOX1 expression level is increased. | Cell Death Discovery

Fig. 1: Ferroptosis occurs after SCIRI and HMOX1 expression level is increased.

From: HMOX1 interacts with BNIP3 to modulate neuronal ferroptosis after spinal cord ischemia-reperfusion injury via a mitophagy-dependent mechanism

Fig. 1: Ferroptosis occurs after SCIRI and HMOX1 expression level is increased.The alternative text for this image may have been generated using AI.

Rat spinal cords were subjected to I/R injury (I: 45 min; R: 12, 24, 48, or 72 h) or sham surgery. A–D Western blotting analysis of ferroptosis-related proteins (ACSL4, FTH1, GPX4) in rat spinal cord tissues (n = 3). E–G qRT-PCR analysis of mRNA expression of ferroptosis-related proteins (n = 3). H, I Determination of relative levels of MDA and GSH (n = 6). J–L Immunofluorescence staining was used to detect the levels of ACSL4 and GPX4 (n = 6). M, O Detection of HMOX1 protein and mRNA levels after SCIRI using western blotting and qRT-PCR (n = 3). P Immunofluorescence assay to detect HMOX1 expression after SCIRI (n = 6). Compared with the sham group, *P < 0.05, **P < 0.01, ***P < 0.001.

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