Fig. 5: HMOX1 is associated with excessive activation of mitophagy. | Cell Death Discovery

Fig. 5: HMOX1 is associated with excessive activation of mitophagy.

From: HMOX1 interacts with BNIP3 to modulate neuronal ferroptosis after spinal cord ischemia-reperfusion injury via a mitophagy-dependent mechanism

Fig. 5: HMOX1 is associated with excessive activation of mitophagy.The alternative text for this image may have been generated using AI.

A, B Western blotting analysis of mitophagy-related proteins (P62, COXIV, TOMM20, LC3) in OGD/R-treated cells (OGD: 6 h; R: 24 h) post-LV-shHMOX1 and LV-HMOX1 transfection (n = 3). C–F Western blotting analysis of mitophagy-related protein expression post-HMOX1 knockdown or overexpression with rapamycin or mdivi-1 intervention (n = 3). G–J Immunofluorescence staining validated P62 expression and TOMM20/LC3 co-localization (n = 6). K, L JC-1 staining analysis of changes in MMP across groups (n = 6). M, N Transmission electron microscopy analysis of autophagosome numbers across groups (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001.

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