Fig. 2: SPOP knockdown promotes CRC progression. | Cell Death Discovery

Fig. 2: SPOP knockdown promotes CRC progression.

From: O-GlcNAcylation of SPOP regulates colorectal cancer progression and ferroptosis by mediating β-catenin degradation

Fig. 2

A Lentiviral vectors expressing SPOP-specific shRNA were used to infect HCT116 and DLD1 cells, and WB analysis was performed to verify the knockdown efficiency. B Cell proliferation in these cell lines was measured using the CCK-8 assay. C Cell cycle phases were analyzed by flow cytometry, with representative images and quantitative results displayed. D, E Transwell assay. F, G Wound healing assay H Invadopodia were visualized by colocalization of cortactin and F-actin. I Representative images of lung sections from HCT116 cells and quantification of metastatic lung nodules; N = 5,scale bars, 500 µm.

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