Fig. 8: The SPOP/β-catenin/SCL7A11 axis regulates CRC ferroptosis. | Cell Death Discovery

Fig. 8: The SPOP/β-catenin/SCL7A11 axis regulates CRC ferroptosis.

From: O-GlcNAcylation of SPOP regulates colorectal cancer progression and ferroptosis by mediating β-catenin degradation

Fig. 8

A ChIP-seq of TCF4 in the promoter region of SLC7A11. B qRT‒PCR was used to detect SLC7A11 mRNA levels in DLD1 cells transfected with shSPOP and SLC7A11 mRNA levels in HCT116 cells overexpressing SPOP. C The mRNA levels of SLC7A11 in HCT116 cells transfected with the β-catenin overexpression plasmid were detected via qRT‒PCR. D–G Dual luciferase reporter assay of SLC7A11 promoter activity. H TCF4-binding motif of SLC7A11 obtained from JASPAR. I ChIP‒qPCR analysis demonstrated the binding of TCF4 to the SLC7A11 promoter. J ChIP‒qPCR results also confirmed the association between β-catenin and the SLC7A11 promoter region. K HCT116 cells were transfected with the SPOP overexpression plasmid, and the cells were collected for WB analysis.

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