Fig. 2: TKT promotes RCC cell migration and invasion in vitro.

A Confirmation of TKT overexpression in 786-O and ACHN cells by western blot. B Wound healing assays indicating that TKT overexpression enhances RCC cell migration. C Confirmation of TKT knockdown in 786-O and ACHN cells by western blot. D Wound healing assays demonstrating that TKT knockdown significantly inhibits wound closure in 786-O and ACHN cells. E Transwell assays revealing that TKT overexpression significantly promotes migration and invasion in 786-O and ACHN cells, with corresponding statistical analyses. F Transwell assays showing that TKT knockdown reduces RCC cell motility, with corresponding statistical analyses. *P < 0.05, **P < 0.01, ***P < 0.001.