Fig. 8: The sensitivity of RCC cells to cisplatin can be enhanced by blocking the TKT-PKM2 axis. | Cell Death Discovery

Fig. 8: The sensitivity of RCC cells to cisplatin can be enhanced by blocking the TKT-PKM2 axis.

From: TKT drives renal cell carcinoma progression through metabolic reprogramming and synergistic interaction with PKM2

Fig. 8: The sensitivity of RCC cells to cisplatin can be enhanced by blocking the TKT-PKM2 axis.The alternative text for this image may have been generated using AI.

A, B CCK-8 assays showing the effect of TKT overexpression on the viability of 786-O and ACHN cells treated with cisplatin at different concentrations. C, D CCK-8 assays demonstrating the effect of TKT knockdown on the viability of 786-O and ACHN cells following treatment with cisplatin at different concentrations. E, F CCK-8 assays illustrating the effect of PKM2 knockdown on the viability of 786-O and ACHN cells upon treatment with cisplatin at different concentrations. G, H CCK-8 assays showing the effect of PKM2 knockdown on the viability of TKT overexpressed 786-O and ACHN cells upon treatment with cisplatin at different concentrations. In 786-O cells, 0.5 µM: Ctrl vs TKT: *; TKT vs TKT+ siPKM2 #1: *; TKT vs TKT+ siPKM2 #2: ***. 1 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. 2.5 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. 5 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. 10 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. In ACHN cells, 0.5 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. 1 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. 2.5 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. 5 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. 10 µM: Ctrl vs TKT: ***; TKT vs TKT+ siPKM2 #1: ***; TKT vs TKT+ siPKM2 #2: ***. *P < 0.05, **P < 0.01, ***P < 0.001.

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