Fig. 1: ChaC1 overexpression sensitizes AUR-induced cell death in HCC cells. | Cell Death Discovery

Fig. 1: ChaC1 overexpression sensitizes AUR-induced cell death in HCC cells.

From: ChaC1-based drug screenings identify a synergistic lethal effect of auranofin and proteasome inhibitors in hepatocellular carcinoma cells

Fig. 1: ChaC1 overexpression sensitizes AUR-induced cell death in HCC cells.The alternative text for this image may have been generated using AI.

A, B Huh7 cells were infected with Ad-NC or Ad-ChaC1 (1×108 pfu/mL). 24 h later, cells were treated with 1 µM drugs from FDA-approved drug library (Plate L1021-19). 24 h later, cell viabilities were measured by CCK8 assay (A). The ratios of cell viabilities of Ad-ChaC1 infected cells to Ad-NC infected cells were calculated and plotted in (B). (C ~ F) Huh7 and Hep3B cells were infected with Ad-NC or Ad-ChaC1 (1×108 pfu/mL) for 24 h. The expression levels of ChaC1 were determined by western blotting (C, D). Intracellular glutathione levels in Huh7 and Hep3B were measured by Tietze’s recycling assay (E, F). G, H Huh7 (G) and Hep3B (H) cells were infected with 1×108 pfu/mL Ad-NC or Ad-ChaC1 for 24 h, and then treated with increasing doses of AUR for 24 h. Cell viabilities were measured by CCK8 assay. I–L Huh7 (I, K) and Hep3B (J, L) cells were infected with adenovirus for 24 h and then treated with 0.3 µM AUR for 24 h. Cell viability was measured by CCK8 assay (I, J). Cell death was measured by propidium iodide staining (1 µg/mL) followed by flow cytometric analysis (K, L).

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